Specifications: 25ug (50ul, 0.5mg/ml) - 50 TEST (100 µl staining volume)
100ug (200ul, 0.5mg/ml) - 200 TEST (100 µl staining volume)
Price: contact us for price information
Product Name: iFluor488 Anti-Mouse CD4 (RM4-5) Serum-Free Monoclonal Antibody
Description: CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Specificity：Against Mouse CD4 Antigen
Application：Flow Cytometry (For Research Purpose Only)
Each lot of this antibody is quality control tested with flow cytometric analysis. The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl. Typically we suggest to use 1:100 titration for staining 1 millions cells in 100 µl staining. Since applications vary, the appropriate dilutions must be determined for individual use.
Figure (Flow Cytometric Analysis): 1 million mouse splenocytes were stained with 0.25µg CD4-iFluor488 or CD4-FITC for comparison. iFluor488 labeled antibody gives stronger signal compared with CD4-FITC.