CD11b Monoclonal Antibody (5C6)

iFluor 488 Anti-Mouse CD11b (Clone: 5C6) Serum-Free Monoclonal Antibody

Catalog:  ZX019R1-025;ZX019R1-100 

Specifications: 25µg (50µl, 0.5mg/ml) - 50 TEST (100 µl staining volume)

                             100µg (200µl, 0.5mg/ml) - 200 TEST  (100 µl staining volume)

Price: contact us for price information

Product Name: iFluor488 Anti-Mouse CD11b (5C6) Monoclonal Antibody

Description: CD11b is integrin ITGAM. Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity). [provided by UniProtKB] 

Specificity:Against Mouse CD11b Antigen

Host:Rat

Application:Flow Cytometry (For Research Purpose Only)

Each lot of this antibody is quality control tested with flow cytometric analysis. The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl. Typically we suggest to use 1:100 titration for staining 1 millions cells in 100 µl staining. Since applications vary, the appropriate dilutions must be determined for individual use.

Figure (Flow Cytometric Analysis): 1 million mouse splenocytes were stained with 0.25µg CD11b-iFluor488 (Purple: staining on CD11b+ monocytes, Red: negative control). iFluor488 labeled antibody gives stronger signal compared with FITC congugated antibodies.

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