Specifications: 25µg (50ul, 0.5mg/ml) - 50 TEST (100 µl staining volume)
100µg (200ul, 0.5mg/ml) - 200 TEST (100 µl staining volume)
Price: contact us for price information
Product Name: FITC Anti-Mouse CD25 (PC61-5.3) Monoclonal Antibody
Description: CD25 is IL2RA. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Specificity：Against Mouse CD25 Antigen
Application：Flow Cytometry (For Research Purpose Only)
Each lot of this antibody is quality control tested with flow cytometric analysis. The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl. Typically we suggest to use 1:100 titration for staining 1 millions cells in 100 µl staining. Since applications vary, the appropriate dilutions must be determined for individual use.
Figure (Flow Cytometric Analysis): 1 million mouse splenocytes were stained with 0.25µg CD25-FITC.