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CD25 Monoclonal Antibody (PC61-5.3)

FITC 488 Anti-Mouse CD25 (Clone: PC61-5.3) Serum-Free Monoclonal Antibody

Catalog:  ZX020R1-025;ZX020R1-100 

Specifications: 25µg (50ul, 0.5mg/ml) - 50 TEST (100 µl staining volume)

                             100µg (200ul, 0.5mg/ml) - 200 TEST  (100 µl staining volume)

Price: contact us for price information

Product Name: FITC Anti-Mouse CD25 (PC61-5.3) Monoclonal Antibody

Description:  CD25 is IL2RA. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] 

Specificity:Against Mouse CD25 Antigen

Host:Rat

Application:Flow Cytometry (For Research Purpose Only)

Each lot of this antibody is quality control tested with flow cytometric analysis. The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl. Typically we suggest to use 1:100 titration for staining 1 millions cells in 100 µl staining. Since applications vary, the appropriate dilutions must be determined for individual use.

Figure (Flow Cytometric Analysis): 1 million mouse splenocytes were stained with 0.25µg CD25-FITC.

Product Manual

ZX020FT 抗鼠CD25流式单抗(FITC)[PC 61 5.3] (pdf)下载

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