CD3 Monoclonal Antibody (145-2C11)

iFluor 488 Anti-Mouse CD3 (Clone: 145-2C11) Serum-Free Monoclonal Antibody

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Catalog:  ZX003R1-025;ZX003R1-100 

Specifications: 25ug (50ul, 0.5mg/ml) - 50 TEST (100 µl staining volume)

                             100ug (200ul, 0.5mg/ml) - 200 TEST  (100 µl staining volume)

Price: contact us for price information

Product Name: iFluor488 Anti-Mouse CD3 (145-2C11) Monoclonal Antibody

Description:  The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]  

Specificity:Against Mouse CD3 Antigen

Host:Rat

Application:Flow Cytometry (For Research Purpose Only)

Each lot of this antibody is quality control tested with flow cytometric analysis. The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl. Typically we suggest to use 1:100 titration for staining 1 millions cells in 100 µl staining. Since applications vary, the appropriate dilutions must be determined for individual use.

Figure (Flow Cytometric Analysis): 1 million mouse splenocytes were stained with 0.25µg CD3-iFluor488 or CD3-FITC for comparison. iFluor488 labeled antibody gives stronger signal compared with CD3-FITC.