Specifications: 25ug (50ul, 0.5mg/ml) - 50 TEST (100 µl staining volume)
100ug (200ul, 0.5mg/ml) - 200 TEST (100 µl staining volume)
Price: contact us for price information
Product Name: iFluor488 Anti-Mouse CD3 (145-2C11) Monoclonal Antibody
Description: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Specificity：Against Mouse CD3 Antigen
Application：Flow Cytometry (For Research Purpose Only)
Each lot of this antibody is quality control tested with flow cytometric analysis. The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl. Typically we suggest to use 1:100 titration for staining 1 millions cells in 100 µl staining. Since applications vary, the appropriate dilutions must be determined for individual use.
Figure (Flow Cytometric Analysis): 1 million mouse splenocytes were stained with 0.25µg CD3-iFluor488 or CD3-FITC for comparison. iFluor488 labeled antibody gives stronger signal compared with CD3-FITC.